Plasmid

Part:BBa_K3600013:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


Lys2-HSP12pr-mScarleti cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1020
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1845
    Illegal XbaI site found at 965
    Illegal XbaI site found at 1274
    Illegal XbaI site found at 1549
    Illegal XbaI site found at 2022
    Illegal SpeI site found at 2861
    Illegal PstI site found at 2870
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1020
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1845
    Illegal SpeI site found at 2861
    Illegal PstI site found at 2870
    Illegal NotI site found at 749
    Illegal NotI site found at 3425
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2592
    Illegal EcoRI site found at 1020
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1845
    Illegal BglII site found at 1567
    Illegal BglII site found at 2463
    Illegal BglII site found at 2885
    Illegal BamHI site found at 2897
    Illegal XhoI site found at 2906
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1020
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1845
    Illegal XbaI site found at 965
    Illegal XbaI site found at 1274
    Illegal XbaI site found at 1549
    Illegal XbaI site found at 2022
    Illegal SpeI site found at 2861
    Illegal PstI site found at 2870
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1020
    Illegal EcoRI site found at 1265
    Illegal EcoRI site found at 1845
    Illegal XbaI site found at 965
    Illegal XbaI site found at 1274
    Illegal XbaI site found at 1549
    Illegal XbaI site found at 2022
    Illegal SpeI site found at 2861
    Illegal PstI site found at 2870
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 782


Design Notes

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Source

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References